Journal: Annals of neurology
Article Title: Mutations in HID1 Cause Syndromic Infantile Encephalopathy and Hypopituitarism
doi: 10.1002/ana.26127
Figure Lengend Snippet: Pituitary Gland. (A) Analysis of the pituitary gland of patient 1:III-3 shows normal adenoid morphology. Staining with antibodies against pituitary hormones depicts moderate ACTH reduction, while FSH, HGH, LH, Prolactin (Prol) and TSH are strongly reduced compared to the control (inlay) (magnification 200×). (B) Immunofluorescence analysis with antibodies against HID1 shows a cytoplasmic expression in neuronal cells of the frontal cortex and cerebellum and in adenoid cells of the pituitary gland in patient and control (magnification ×630, white bars correspond 50 μm).
Article Snippet: Immunohistochemical analysis was performed at paraffin sections from the pituitary gland using a Bench Mark XT automatic staining platform (Ventana, Heidelberg, Germany; ultraview Universal DAB detection kit) with the following primary antibodies: mouse anti-ACTH (Adrenocorticotrophic Hormone) (DAKO M3501 (02A39); 1:2000); mouse anti-FSH (Follicle Stimulating Hormone) (Zytomed 5063134 (SPM107); 1:2000), rabbit anti-HGH (Human Growth Hormone) (DCS G1100C002, 1:200), mouse anti-LH (Luteinizing Hormone), Zytomed 512–3094 (SPM103), 1:1500), mouse anti-Prolactin (Biogenix MU031-UC,1:300), mouse anti-TSH (Thyroid Stimulating Hormone) (Dako M3503 (0042), 1:500).
Techniques: Staining, Immunofluorescence, Expressing